Expression of a Chinese Cabbage Cysteine Proteinase Inhibitor, BrCYS1, Retards Seed Germination and Plant Growth in Transgenic Tobacco Plant

Abstract

Phytocystatins are plant cysteine proteinase inhibitors that regulate endogenous and heterologous cysteine proteinases of the papain family. A cDNA encoding the phytocystatin RrCYS1 (Brassica rapa cysteine proteinase inhibitor 1) has been isolated from Chinese cabbage (B. rapa subsp. pekinensis) flower buds. In order to explore the role of this inhibitory enzyme, tobacco plants (Nicotiana tabacum L. cv. Samson) containing altered amounts of phytocystatin were generated by over-expressing BrCYS1 cDNA in either the sense or the antisense configuration. The resulting plants had in vitro enzyme inhibitory activities that were over 10% of those detected in wild type plants. The transgenic plants exhibited retarded seed germination and seedling growth and a reduced seed yield, whereas these properties were enhanced in antisense plants. These data suggest that BrCYS1 participates in the control of seed germination, post-germination and plant growth by regulating cysteine peptidase activity.