In vitro system에서 청국장 메탄올 추출물의 신경세포 보호효과

Abstract

In this study, the neuronal cell protective effects of methanol extract from cheonggukjang were evaluated. The proximate composition and total phenolics of the methanol extract were 40.95% crude protein, 22.49% crude fat, 15.99% nitrogen free extract, 7.91% moisture, 6.74% crude ash, 5.92% crude fiber, and 28.43 mg/g of total phenolics. Intracellular ROS accumulation resulting from H2O2 treatment of PC12 cells was significantly reduced when methanol extract was present in the media compared to PC12 cells treated with H2O2 only. In a cell viability assay using 3-(4,5-dimethylthiazol- 2-yl)-2,5-diphenyl-tetrazolium-bromide (MTT), the methanol extract showed protective effects against H2O 2-induced neurotoxicity, and lactate dehydrogenase (LDH) release into the medium was also inhibited. Furthermore, the inhibitory effect of the methanol extract against acetylcholinesterase was dose-dependent. ? The Korean Society of Food Science and Technology.