안토시아닌이 Vascular Endothelial Growth Factor 매개의 혈관형성에 미치는 영향에 대한 연구

Abstract

Angiogenesis, the process of new blood vessel growth from pre-existing capillaries, is involved in physiological and pathological processes such as tumor growth and metastasis. Thus, suppression of abnormal angiogenesis may provide therapeutic strategies for the treatment of angiogenesis-dependent disorders, like as skin cancer, psoriasis, and other reactive skin disorders. There is increasing evidence that polyphenols found in natural products may have a beneficial preventive effect on cardiovascular disease and cancer by mechanisms including factor of angiogneisis that have not yet been elucidated. In the present study, we investigated the antiangiogenic activities of anthocyanin, a class of polyphenols present at high levels in dietary black bean, in both in vitro and in vivo models. In vitro studies were performed using cultured human umbilical vein endothelial cells (HUVEC). After inducing angiogenesis by vascular endothelial cell growth factor (VEGF) on those cultured cells, we analyzed dose-dependent changes before- and after-anthocyanin treatment. In vivo, Chorioallantoic membrane (CAM) assay was done to check developmental effect on embryo vasculature. Dietary anthocyanin from black bean had statistically significant inhibitions on endothelial cell migration and tube formation in dose dependant manner, and also inhibited embryological vascular development. We suggest that an anthocyanin derived from diet has a natural VEGF inhibitor that could potentially be useful in cancer prevention and/or treatment. (Cancer Prev Res 15, 60-68, 2010)