Proteomic Analysis of Focal Cerebral Ischemic Injury in Male Ratsopen access
- Authors
- Koh, Phil-Ok
- Issue Date
- Feb-2010
- Publisher
- JAPAN SOC VET SCI
- Keywords
- brain ischemia; MCAO; proteomics
- Citation
- JOURNAL OF VETERINARY MEDICAL SCIENCE, v.72, no.2, pp.181 - 185
- Indexed
- SCIE
SCOPUS
- Journal Title
- JOURNAL OF VETERINARY MEDICAL SCIENCE
- Volume
- 72
- Number
- 2
- Start Page
- 181
- End Page
- 185
- URI
- https://scholarworks.bwise.kr/gnu/handle/sw.gnu/25243
- DOI
- 10.1292/jvms.09-0364
- ISSN
- 0916-7250
- Abstract
- The present study identified the proteins that are differentially expressed during ischemic brain injury. Adult male rats were performed a middle cerebral artery occlusion (MCAO) to induce cerebral ischemia, and brains were collected at 24 hr after MCAO. Protein analysis was performed on the cerebral cortex using two-dimensional gel electrophoresis. Protein spots with a greater than 3 fold change in intensity between the sham and MCAO groups were identified by mass spectrometry. Among these proteins, 60 kDa heat shock protein, dehydropyrimidinase-related protein 2, t-complex protein 1, and Rho GDP dissociation inhibitor levels were significantly increased in MCAO group compared to those of the sham group. In contrast, thioredoxin, peroxiredoxin-2, stathmin, ubiquitin carboxyterminal hydrolase L1, guanine nucleotide-binding protein a, pyridoxal-5'-phosphate phosphatase, and apoplipoprotein A-I levels were significantly decreased in MCAO group. These results suggest that cerebral ischemia induces neuronal cells death by changing expression levels of several proteins.
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