Production of Tropane Alkaloids during De-differentiation of Scopolia parviflora Calli

Abstract

The production of tropane alkaloids, during differentiation and de-differentiation of Scopolia parviflora calli was Studied. Tropane alkaloid production drastically decreased during calli de-differentiation. Scopolamine (1) production decreased after 10 days Of Culture, whereas that of hyoscyamine (2) decreased during de-differentiation of root to calli. The production of I was enhanced in Calli Undergoing differentiation to shoot after 60 days of Culture, reaching a maximum by 80 days. However, production of hyoscyamine in regenerated plants was lower. The expression level of hyoscyamine 6 beta-hydroxylase (H6H), a key biosynthetic enzyme for tropane alkaloids. was significantly increased in 4-week-old calli. This study suggests that the biosynthesis of tropane alkaloids is regulated inversely in de-differentiating Scopolia parviflora calli.