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Cloning of aprE86-1 Gene Encoding a 27-kDa Mature Fibrinolytic Enzyme from Bacillus amyloliquefaciens CH86-1
- Authors
- Lee, Ae Ran; Kim, Gyoung Min; Kwon, Gun-Hee; Lee, Kang Wook; Park, Jae-Yong; Chun, Jiyeon; Cha, Jaeho; Song, Young-Sun; Kim, Jeong Hwan
- Issue Date
- Feb-2010
- Publisher
- KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY
- Keywords
- Bacillus amyloliquefaciens; fibrinolytic enzymes; gene expression
- Citation
- JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, v.20, no.2, pp 370 - 374
- Pages
- 5
- Indexed
- SCIE
SCOPUS
KCI
- Journal Title
- JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
- Volume
- 20
- Number
- 2
- Start Page
- 370
- End Page
- 374
- ISSN
- 1017-7825
1738-8872
- Abstract
- A gene encoding the major secreted fibrinolytic protein of Bacillus amyloliquefaciens CH86-1 was cloned from genomic DNAs. DNA sequencing showed that the gene, aprE86-1, could direct the synthesis of a mature protein 275 amino acids in length after processing. When aprE86-1 was introduced into B. subtilis, a mature 27-kDa protein was produced as expected. The fibrinolytic activity of the B. subtilis transformant (TF) was higher than that of B. amyloliquefaciens CH86-1, showing the possibility of increasing the fibrinolytic activity of Bacillus strains through genetic engineering.
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