Generation of a recloned transgenic cat expressing red fluorescence protein
- Authors
- Cho, S. J.; Bang, J. I.; Yu, X. F.; Lee, Y. S.; Kim, J. H.; Jeon, J. T.; Yee, S. T.; Kong, I. K.
- Issue Date
- 15-Apr-2010
- Publisher
- ELSEVIER SCIENCE INC
- Keywords
- Cloning; Recloned RFP transgenic cat; Red fluorescence protein; SCNT
- Citation
- THERIOGENOLOGY, v.73, no.7, pp.848 - 855
- Indexed
- SCIE
SCOPUS
- Journal Title
- THERIOGENOLOGY
- Volume
- 73
- Number
- 7
- Start Page
- 848
- End Page
- 855
- URI
- https://scholarworks.bwise.kr/gnu/handle/sw.gnu/25136
- DOI
- 10.1016/j.theriogenology.2009.09.008
- ISSN
- 0093-691X
- Abstract
- Somatic cells from a first-generation red fluorescence protein transgenic cat (first RFP TG cat) were used to produce a recloned RFP transgenic cat (Re-RFP TG cat) (Felis catus) that systemically expressed RFP. A total of 281 RFP cloned embryos were transferred into 13 surrogate mothers (mean = 21 +/- 7.7 embryos/recipient). One surrogate cat was diagnosed pregnant (7.7%) and delivered one live kitten. The presence of the RFP gene in the mRNA and genomic DNA of the Re-RFP TG cat was confirmed by polymerase chain reaction analyses, and red fluorescence was detected in its internal organs and placental tissue samples. Analysis of nine feline-specific microsatellite loci confirmed that the Re-RFP TG cat was genetically identical to the donor cat. To test whether results such as normality of offspring and a low cloning success were due to epigenetic modifications, global methylation of placenta from the two first cloned RFP TG cats (77.08% and 82.29%) and the Re-RFP TG cat (76.38%) were compared by bisulfite mutagenesis sequencing analysis. In conclusion, although cloning efficiency was low, we demonstrated the successful use of a cloned first RFP TG cat as a donor cat to produce a Re-RFP TG cat. These results may facilitate future developments in biomedical models for human therapeutic applications. (C) 2010 Elsevier Inc. All rights reserved.
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