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Molecular Cloning of Cyanobacterial Pteridine Glycosyltransferases That Catalyze the Transfer of either Glucose or Xylose to Tetrahydrobiopterinopen access
- Issue Date
- Nov-2010
- Publisher
- AMER SOC MICROBIOLOGY
- Citation
- APPLIED AND ENVIRONMENTAL MICROBIOLOGY, v.76, no.22, pp 7658 - 7661
- Pages
- 4
- Indexed
- SCI
SCIE
SCOPUS
- Journal Title
- APPLIED AND ENVIRONMENTAL MICROBIOLOGY
- Volume
- 76
- Number
- 22
- Start Page
- 7658
- End Page
- 7661
- ISSN
- 0099-2240
1098-5336
- Abstract
- Here, we report cloning of cyanobacterial genes encoding pteridine glycosyltransferases that catalyze glucosyl or xylosyl transfer from UDP-sugars to tetrahydrobiopterin. The genes were cloned by PCR amplification from genomic DNA which was isolated from culture and environmental samples and overexpressed in Escherichia coli for an in vitro activity assay.
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