Proteomic Analysis of Effects on Natural Herb Additive Containing Immunoglobulin Yolksac (IgY) in Pigs
- Authors
- Park, Hyeon-Soo; Park, Kwang-Il; Nagappan, Arulkumar; Lee, Do-Hoon; Kang, Sang-Rim; Kim, Jin-A; Kim, Eun Hee; Han, Dae-Yong; Kim, Gon-Sup
- Issue Date
- 2011
- Publisher
- WORLD SCIENTIFIC PUBL CO PTE LTD
- Keywords
- Natural Herb; Immunoglobulin Yolksac (IgY); Glutamic Pyruvic Transaminase (GPT); Glutamic Oxaloacetic Transaminase (GOT); Proteome
- Citation
- AMERICAN JOURNAL OF CHINESE MEDICINE, v.39, no.3, pp.477 - 488
- Indexed
- SCIE
SCOPUS
- Journal Title
- AMERICAN JOURNAL OF CHINESE MEDICINE
- Volume
- 39
- Number
- 3
- Start Page
- 477
- End Page
- 488
- URI
- https://scholarworks.bwise.kr/gnu/handle/sw.gnu/24798
- DOI
- 10.1142/S0192415X1100897X
- ISSN
- 0192-415X
- Abstract
- Thirty male pigs were infected orally with E. coli and Salmonella typhimurium, and divided into a control group and two additive groups to determine the effect of an additive mixture on the changes in protein expression. The pigs were given a food supplemented with a natural herbal additive containing immunoglobulin yolksac (IgY) at concentrations of 0.5% or 1%. On the 1st day and after eight weeks of feeding, the body weight gain, food intake and serum GOT/GPT levels were examined. The GOT/GPT levels on the 1st day were similar in the three groups. However, after eight weeks of feeding, the GOT level was significantly lower in the additive treatment groups (0.5% and 1.0%). In addition, the changes in the spleen proteome as a response to the herbal additive were examined using two-dimensional polyacrylamide gel electrophoresis. A total of 31 differentially expressed protein spots were identified by comparing the protein profiles of the control and additive treated porcine spleens. Finally, 19 proteins were detected by MALDI-TOF/MS. Overall, the proteins detected are involved in a range of biological process, such as metabolic processes, biological processes, transport, carbohydrate metabolic processes, generation of precursors and energy. In conclusion, these results support of the hypothesis that a natural herbal additive containing IgY can affect the immune regulation system and reduce the stress of microbial infections.
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Collections - 수의과대학 > Department of Veterinary Medicine > Journal Articles
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