시판 메밀차 열수 추출물의 항산화 및 신경세포 보호효과

Abstract

The antioxidant and neuronal cell-protective effects of hot water extract from commercial buckwheat tea (CBTE) were evaluated. The 2,2'-azino-bis(3-ethyl-benzthiazoline-6-sulfonic acid) (ABTS) radical scavenging activity, ferric reducing antioxidant power (FRAP), and malondialdehyde (MDA) inhibitory effect of the CBTE increased in a dose-dependent manner. The Intracellular reactive oxygen species (ROS) accumulation that resulted from hydrogen peroxide (H2O2) treatment more significantly decreased when CBTE was present in the media than when the PC12 cells were treated only with H2O2. In the neuronal cell viability assay using 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyl-tetrazoliumbromide (MTT), the aqueous extracts showed a protective effect against H2O2-induced neurotoxicity, and the lactate dehydrogenase (LDH) release into the medium was also inhibited by CBTE. The total phenolics of CBTE was 9,608.10 mg/100 g, and the major phenolic compounds were rutin (13.42 mg/100 g) and quercitrin (0.90 mg/100 g). These data suggested that CBTE, including the aforementioned phenolics, may be useful in reducing the risk of neurodegenerative disease.