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A simple and efficient method for isolation of a single Eimeria oocyst from poultry litter using a micromanipulator
- Issue Date
- Apr-2011
- Publisher
- ELSEVIER SCI LTD
- Keywords
- Chickens; Eimeria isolation method; Micromanipulator; Poultry
- Citation
- RESEARCH IN VETERINARY SCIENCE, v.90, no.2, pp 260 - 261
- Pages
- 2
- Indexed
- SCI
SCIE
SCOPUS
- Journal Title
- RESEARCH IN VETERINARY SCIENCE
- Volume
- 90
- Number
- 2
- Start Page
- 260
- End Page
- 261
- ISSN
- 0034-5288
1532-2661
- Abstract
- The chicken, which is the host for seven species of Eimeria, typically is infected simultaneously by multiple Eimeria species and the oocysts of coccidia are excreted in the feces. A prerequisite for investigation of individual Eimeria species is to isolate a single oocyst from fecal samples. A novel method for isolating a single Eimeria oocyst from poultry litter using a micromanipulator was developed. This simple method is fast and reliable, and provides direct isolation of a single sporulated oocyst from fecal samples harboring multiple Eimeria species or samples contaminated by other species of parasite. (C) 2010 Elsevier Ltd. All rights reserved.
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