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Cloning and Expression of a bpr Gene Encoding Bacillopeptidase F from Bacillus amyloliquefaciens CH86-1
- Authors
- Kwon, Gun-Hee; Park, Jae-Yong; Kim, Jong-Sang; Lim, Jinkyu; Park, Cheon-Seok; Kwon, Dae Young; Kim, Jeong Hwan
- Issue Date
- May-2011
- Publisher
- KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY
- Keywords
- Bacillus amyloliquefaciens CH86-1; bacillopeptidase F; bpr gene; fibrinolytic enzymes
- Citation
- JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, v.21, no.5, pp 515 - 518
- Pages
- 4
- Indexed
- SCIE
SCOPUS
KCI
- Journal Title
- JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
- Volume
- 21
- Number
- 5
- Start Page
- 515
- End Page
- 518
- ISSN
- 1017-7825
1738-8872
- Abstract
- A gene encoding bacillopeptidase F, bpr86-1, was cloned from B. amyloliquefaciens CH86-1 isolated from cheonggukjang. This gene could encode a preproenzyme of 1,431 amino acids. When bpr86-1 was introduced into B. subtilis WB600 via pHY300PLK, an E. coli Bacillus shuttle vector, the transformant showed fibrinolytic activity. During growth on LB, the fibrinolytic activity of cells increased sharply when they entered the stationary phase. The highest activity (761.4 mU/mg protein) was observed at 96 h of cultivation.
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