RGS2-Mediated Intracellular Ca2+ Level Plays a Key Role in the Intracellular Replication of Brucella abortus Within Phagocytesopen access
- Authors
- Kim, Dong Hyeok; Lim, Jeong Ju; Lee, Jin Ju; Kim, Dae Geun; Lee, Hu Jang; Min, Wongi; Kim, Kwang Dong; Chang, Hong Hee; Endale, Mehari; Rhee, Man Hee; Watarai, Masahisa; Kim, Suk
- Issue Date
- 1-Feb-2012
- Publisher
- OXFORD UNIV PRESS INC
- Citation
- JOURNAL OF INFECTIOUS DISEASES, v.205, no.3, pp.445 - 452
- Indexed
- SCIE
SCOPUS
- Journal Title
- JOURNAL OF INFECTIOUS DISEASES
- Volume
- 205
- Number
- 3
- Start Page
- 445
- End Page
- 452
- URI
- https://scholarworks.bwise.kr/gnu/handle/sw.gnu/22326
- DOI
- 10.1093/infdis/jir765
- ISSN
- 0022-1899
- Abstract
- Background. Brucella abortus can proliferate within professional and nonprofessional phagocytic host cells and thereby successfully bypass the bacteriocidal effects of phagocytes. However, the intracellular survival mechanism and factors of virulence are not fully understood. Methods. We have investigated the role of the regulator of G protein signaling 2 (RGS2), an intracellular calcium ([Ca2+](i)) regulator of the host cell, in the intracellular survival of B. abortus within phagocytes. Results. B. abortus infection markedly induced RGS2 messenger RNA expression in early phase and increased the [Ca2+](i) level up to 24 hours postinfection within macrophages from wild-type mice. The [Ca2+](i) level, however, was not influenced by B. abortus infection within macrophages from RGS2-deficient mice. Furthermore, B. abortus survival was reduced within RGS2-deficient macrophages, and hence bacterial proliferation was inhibited in RGS2-deficient mice. Moreover, treatment with the Ca2+ chelator ethylenediaminetetraacetic acid (EDTA) or 1,2-bis-(2-amino-phenoxy)ethane-N,N,N',N'-tetraacetic acid acetoxymethyl ester (BAPTA-AM) and the L-type Ca2+ channel-blocking agent nifedipine or genistein also showed a reduced intracellular replication of B. abortus within macrophages. Conclusion. These results indicate that B. abortus infection induces host RGS2 expression and that up-regulation of [Ca2+](i) levels is an essential factor for the intracellular survival of B. abortus within phagocytes.
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Collections - 농업생명과학대학 > 축산과학부 > Journal Articles
- 수의과대학 > Department of Veterinary Medicine > Journal Articles
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