Identification of genes contributing to the intracellular replication of Brucella abortus within HeLa and RAW 264.7 cells
- Authors
- Kim, Dong Hyeok; Lim, Jeong Ju; Lee, Jin Ju; Kim, Dae Geun; Lee, Hu Jang; Min, Wongi; Kim, Kwang Dong; Chang, Hong Hee; Rhee, Man Hee; Watarai, Masahisa; Kim, Suk
- Issue Date
- 17-Aug-2012
- Publisher
- ELSEVIER SCIENCE BV
- Keywords
- Brucella abortus; Phagocytes; Virulence associated genes; Mutant
- Citation
- VETERINARY MICROBIOLOGY, v.158, no.3-4, pp.322 - 328
- Indexed
- SCIE
SCOPUS
- Journal Title
- VETERINARY MICROBIOLOGY
- Volume
- 158
- Number
- 3-4
- Start Page
- 322
- End Page
- 328
- URI
- https://scholarworks.bwise.kr/gnu/handle/sw.gnu/22072
- DOI
- 10.1016/j.vetmic.2012.02.019
- ISSN
- 0378-1135
- Abstract
- Brucella abort-us, the causative agent of brucellosis, can survive and replicate within host cells. Understanding bacterial virulence factors and bacteria-host cell interactions is critical for controlling brucellosis, yet very little is known about the virulence strategies and signaling pathways activated in phagocytes during infection to ensure their growth and survival. B. abortus was mutagenized by mini-Tn5Km2 transposon mutagenesis to identify virulence genes related to the internalization and intracellular replication of the bacteria. Of the total 2300 mutants used to infect HeLa cells, 23 mutants defective for intercellular growth and the mutated genes were identified. Sequence analysis of DNA flanking the transposon showed various insertion sites in bacterial genes that might be associated with virulence, including genes associated with lipoproteins, amino acid metabolism, translation, transcription, carbohydrate transport, coenzyme transport, inorganic ion transport, energy metabolism, membrane transport, and cell wall/membrane biogenesis. Moreover, mutants were classified into class I, class II and class III as higher, similar, and lower internalization, respectively, into HeLa cells. Furthermore, defective mutants for intracellular growth in HeLa cells were found to be defective in RAW 264.7 cells. Taken together, we suggest that the identified virulence associated genes might contribute to the intracellular growth and survival of B. abortus in phagocytes. (C) 2012 Elsevier B.V. All rights reserved.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - 농업생명과학대학 > 축산과학부 > Journal Articles
- 수의과대학 > Department of Veterinary Medicine > Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.