Neurogenic and cardiomyogenic differentiation of mesenchymal stem cells isolated from minipig bone marrow
- Authors
- Kumar, B. Mohana; Maeng, Geun-Ho; Lee, Yeon-Mi; Kim, Tae-Ho; Lee, Jeong-Hyeon; Jeon, Byeong-Gyun; Ock, Sun-A; Yoo, Jae-Gyu; Rho, Gyu-Jin
- Issue Date
- Oct-2012
- Publisher
- ELSEVIER SCI LTD
- Keywords
- Mesenchymal stem cells; In vitro differentiation; Neuron-like cells; Cardiomyocyte-like cells; Minipig
- Citation
- RESEARCH IN VETERINARY SCIENCE, v.93, no.2, pp.749 - 757
- Indexed
- SCIE
SCOPUS
- Journal Title
- RESEARCH IN VETERINARY SCIENCE
- Volume
- 93
- Number
- 2
- Start Page
- 749
- End Page
- 757
- URI
- https://scholarworks.bwise.kr/gnu/handle/sw.gnu/21981
- DOI
- 10.1016/j.rvsc.2011.09.012
- ISSN
- 0034-5288
- Abstract
- The present study investigated the potential of minipig bone marrow-mesenchymal stem cells (BM-MSCs) to differentiate in vitro into neuron- and cardiomyocyte-like cells. Isolated BM-MSCs exhibited a fibroblast-like morphology, expressed CD29, CD44 and CD90, and differentiated into osteocytes, adipocytes and chondrocytes. Upon induction in two different neuronal specific media, most of BM-MSCs acquired the distinctive morphological features and positively stained for nestin, neurofilament-M (NF-M), neuronal nuclei (NeuN), beta-tubulin, galactocerebroside (Gal-C) and glial fibrillary acidic protein (GFAP). Expression of nestin, GFAP and NF-M was further demonstrated by RT-PCR and RT-qPCR. Following cardiomyogenic induction, MSCs exhibited a stick-like morphology with extended cytoplasmic processes, and formed cluster-like structures. The expression of cardiac specific markers a-smooth muscle actin, cardiac troponin T, desmin and alpha-cardiac actin was positive for immunofluorescence staining, and further confirmed by RT-PCR and RT-qPCR. In conclusion, our results showed the in vitro differentiation ability of porcine BM-MSCs into neuron-like and cardiomyocyte-like cells. (c) 2011 Elsevier Ltd. All rights reserved.
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