Chicken IL-17F: Identification and comparative expression analysis in Eimeria-infected chickens
- Authors
- Kim, Woo H.; Jeong, Jipseol; Park, Ae R.; Yim, Dongjean; Kim, Yong-Hwan; Kim, Kwang D.; Chang, Hong H.; Lillehoj, Hyun S.; Lee, Byung-Hyung; Min, Wongi
- Issue Date
- Nov-2012
- Publisher
- ELSEVIER SCI LTD
- Keywords
- Chicken; Cytokines; Eimeria infections; Interleukin (IL)-17F
- Citation
- DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY, v.38, no.3, pp.401 - 409
- Indexed
- SCIE
SCOPUS
- Journal Title
- DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
- Volume
- 38
- Number
- 3
- Start Page
- 401
- End Page
- 409
- URI
- https://scholarworks.bwise.kr/gnu/handle/sw.gnu/21934
- DOI
- 10.1016/j.dci.2012.08.002
- ISSN
- 0145-305X
- Abstract
- Interleukin-17F (IL-17F) is a proinflammatory cytokine, which plays an important role in gut homeostasis. A full-length chicken IL-17F (chIL-17F) cDNA with a 510-bp coding region was identified from ConA-activated chicken splenic lymphocytes. ChIL-17F shares 53% amino acid sequence identity with the previously described chicken IL-17 (chIL-17A) and 38-43% with mammalian homologues. The locus harboring chIL-17 and chIL-17F displayed inverted order compared to those of mammals. ChIL-17F transcript expression was high in lymphoblast cell line CU205 and at moderate levels in small and large intestines and liver. ChIL-17F and chIL-17 expression profiles were examined by quantitative real-time RT-PCR in mitogen-stimulated splenic lymphocytes and intestinal areas affected by Eimeria maxima and Eimeria tenella infections. Expression levels of chIL-17F, like chIL-17, were elevated in mitogen-activated splenic lymphocytes. ChIL-17F, but not chIL-17, expression was upregulated in intestinal tissues affected by E. maxima and E. tenella infections. Recombinant chIL-17F biological activities were similar to that of chIL-17 in primary chicken embryonic fibroblasts. These results suggest that chIL-17F is a unique member of the IL-17 family of cytokines. (C) 2012 Elsevier Ltd. All rights reserved.
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