갈색 느티만가닥버섯 메탄올 추출물의 항산화 및 tyrosinase 저해 효과

Abstract

The objective of this study was to evaluate antioxidant effect and tyrosinase inhibitory activity of methanolextracts from Hypsizygus marmoreus. The Hypsizygus marmoreus was divided into two parts (pileus and stipe) andextracted with methanol. Total polyphenolics and flavonoids in the methanol extracts were measured by spectrophotometricmethods and 2,2-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activities have been determined for antioxidantactivities. The total polyphenolics and flavonoids contents of methanol extract of the pileus were higher than methanolextract of the stipes. The total polyphenolics contents in methanol extracts of the pileus and stipes were 8.7 ug/mg and5.6 ug/mg, respectively. The total flavonoids contents in methanol extracts of the pileus and stipes were 2.8 ug/mg and1.4 ug/mg, respectively. The tyrosinase inhibitory activity was proportional to concentration of methanol extract. The tyrosinaseinhibitory activity of the methanol extract (220 mg/ml) of pileus (66.9%) and stipe (57.97%) was lower than thoseof positive control 2% arbutin. The DPPH radical scavenging activity of the methanol extract (20 mg/ml) of pileus andstipes was 52.55% and 30.35%, respectively. Moreover, the effects of methanol extarcts on cell proliferation of B16BL6mouse melanoma cells were investigated using WST-1 assay (4-[3-(4-iodophenyl)-2-(4-nitrophenyl)-2H-5-tetrazolio]-1,3-benzene disulphonate) and B16BL6 mouse melanoma cells treated with methanol extract of 200-2,000 ug/ml were higherproliferation rate than those of 0.04% adenosine