Anthocyanins Downregulate Lipopolysaccharide-Induced Inflammatory Responses in BV2 Microglial Cells by Suppressing the NF-kappa B and Akt/MAPKs Signaling Pathwaysopen access
- Authors
- Jeong, Jin-Woo; Lee, Won Sup; Shin, Sung Chul; Kim, Gi-Young; Choi, Byung Tae; Choi, Yung Hyun
- Issue Date
- Jan-2013
- Publisher
- MDPI
- Keywords
- anthocyanins; BV2; anti-inflammatory activity; NF-kappa B; MAPK; Akt
- Citation
- INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v.14, no.1, pp.1502 - 1515
- Indexed
- SCIE
SCOPUS
- Journal Title
- INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
- Volume
- 14
- Number
- 1
- Start Page
- 1502
- End Page
- 1515
- URI
- https://scholarworks.bwise.kr/gnu/handle/sw.gnu/20858
- DOI
- 10.3390/ijms14011502
- ISSN
- 1661-6596
- Abstract
- Anthocyanins are naturally occurring polyphenols that impart bright color to fruits, vegetables and plants and have a variety of protective properties, which have generally been attributed to their antioxidant capacity. However, little is known about the molecular mechanisms underlying anti-inflammatory effects of anthocyanins related to neurodegenerative diseases. Therefore, we determined whether anthocyanins isolated from black soybean seed coats would inhibit pro-inflammatory mediators and cytokines in lipopolysaccharide (LPS)-stimulated murine BV2 microglial cells. Our results showed that anthocyanins significantly inhibited LPS-induced pro-inflammatory mediators, such as nitric oxide (NO) and prostaglandin E-2, and pro-inflammatory cytokines including tumor necrosis factor (TNF)-alpha and interleukin (IL)-1 beta, without significant cytotoxicity. Anthocyanins also downregulated excessive expression of inducible NO synthase, cyclooxygenase-2, TNF-alpha, and IL-1 beta in LPS-stimulated BV2 cells. Moreover, anthocyanins inhibited nuclear translocation of nuclear factor-kappa B (NF-kappa B) by reducing inhibitor of NF-kappa B alpha degradation as well as phosphorylating extracellular signal-regulated kinase, c-Jun N-terminal kinase, p38 mitogen-activated protein kinase, and Akt. These findings suggest that anthocyanins may offer substantial therapeutic potential for treating inflammatory and neurodegenerative diseases accompanied by microglial activation.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - College of Medicine > Department of Medicine > Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.gnu.ac.kr/handle/sw.gnu/20858)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.