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Variable domain antibodies specific for viral hemorrhagic septicemia virus (VHSV) selected from a randomized IgNAR phage display library
- Authors
- Ohtani, Maki; Hikima, Jun-ichi; Jung, Tae-Sung; Kondo, Hidehiro; Hirono, Ikuo; Takeyama, Haniko; Aoki, Takashi
- Issue Date
- Feb-2013
- Publisher
- Academic Press
- Keywords
- IgNAR; Neutralization activity; Phage display library; VHSV
- Citation
- Fish and Shellfish Immunology, v.34, no.2, pp 724 - 728
- Pages
- 5
- Indexed
- SCI
SCIE
SCOPUS
- Journal Title
- Fish and Shellfish Immunology
- Volume
- 34
- Number
- 2
- Start Page
- 724
- End Page
- 728
- ISSN
- 1050-4648
1095-9947
- Abstract
- Phage display libraries are used to screen for nucleotide sequences that encode immunoglobulin variable (V) regions that are specific for a target antigen. We previously constructed an immunoglobulin new antigen receptor (IgNAR) phage display library. Here we used this library to obtain an IgNAR V region that is specific for viral hemorrhagic septicemia virus (VHSV). A phage clone (clone 653) was found to be specific for VHSV by the biopanning method. The V region of clone 653 was used to construct a 6 x His tagged recombinant IgNAR-653 V protein (rIgNAR-653) using the Escherichia coli pET system. The rIgNAR-653 protein bound specifically to VHSV, confirming its activity. (C) 2012 Elsevier Ltd. All rights reserved.
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