Dynamic methylation pattern of the methyltransferase1o (Dnmt1o) 5 '-flanking region during mouse oogenesis and spermatogenesis
- Authors
- Ko, Yeoung-Gyu; Yun, Jisoo; Park, Hyoung Joon; Tanaka, Satoshi; Shiota, Kunio; Cho, Jae-Hyeon
- Issue Date
- Mar-2013
- Publisher
- WILEY-BLACKWELL
- Keywords
- methyltransferase1o (Dnmt1o); DNA methylation; oogenesis; spermatogenesis
- Citation
- MOLECULAR REPRODUCTION AND DEVELOPMENT, v.80, no.3, pp.212 - 222
- Indexed
- SCIE
SCOPUS
- Journal Title
- MOLECULAR REPRODUCTION AND DEVELOPMENT
- Volume
- 80
- Number
- 3
- Start Page
- 212
- End Page
- 222
- URI
- https://scholarworks.bwise.kr/gnu/handle/sw.gnu/20763
- DOI
- 10.1002/mrd.22153
- ISSN
- 1040-452X
- Abstract
- DNA methyltransferase1o (Dnmt1o), which is specific to oocyte and preimplantation embryo, plays a role in maintaining DNA methylation in mammalian cells. Here, we investigated the methylation status of CpGs sites in the Dnmt1o 5-flanking region in germ cells at different stages of oogenesis or spermatogenesis. The methylation levels of the CpG sites at the 5-flanking regions were hypermethylated in growing oocytes of all follicular stages, while the oocytes in meiotic metaphase II (MII) were demethylated. The methylation pattern within the CpGs sites in the 5-flanking region, however, was dramatically changed during spermatogenesis. We observed that there was significant non-CpG methylation both in MII oocytes and spermatocytes. Although a low methylation level in non-CpG sites was observed in primary and secondary oocytes, the CpA site of position 25 and CpT site of position 29 within the no-CpG region in the 5-flanking region of Dnmt1o was highly methylated in MII oocytes. During spermatogenesis, the low degree of methylation at CpG sites in spermatocytes increased to a higher degree in sperm, while the high ratio of methylation in non-CpG sites in spermatocytes decreased. Together, germ cells showed inverted methylation patterns between CpG and non-CpG sites in the Dnmt1o 5-upstream region, and the methylation pattern during oogenesis did not drastically change, remaining generally hypomethylated at the MII stage. Mol. Reprod. Dev. 80: 212222, 2013. (c) 2013 Wiley Periodicals, Inc.
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