상세 보기
- Wang, Chonglong;
- Zhou, Jia;
- Jang, Hui-Jeong;
- Yoon, Sang-Hwal;
- Kim, Jae-Yean;
- ... Kim, Seon-Won;
- 외 2명
WEB OF SCIENCE
22SCOPUS
25초록
Production of Z-type farnesyl diphosphate (FPP) has not been repotted in Escherichia coli. Here we present the fusion enzyme (ILRv) of E. coli E,E-FPP synthase (IspA) and Mycobacterium tuberculosis Z,E-FPP synthase (Rv1086), which can produce primarily Z,E-FPP rather than E,E-FPP, the predominant stereoisomer found in most organisms. Z,E-farnesol (FOH) was produced from E. coli harboring the bottom portion of the MVA pathway and the fusion FPP synthase (ILRv) at a titer of 115.6 mg/L in 2 YT medium containing 1% (v/v) glycerol as a carbon source and 5 mM mevalonate. The Z,E-FOH production was improved by 15-fold, compared with 7.7 mg/L obtained from the co-overexpression of separate IspA and Rv1086. The Z,E-FPP was not metabolized in native metabolic pathways of E. coli. It would be of interest to produce Z,E-FPP derived sesquiterpenes from recombinant E. coli due to no loss of Z,E-FPP substrate in endogenous metabolism of the host strain. (C) 2013 Elsevier Inc. All rights reserved.
키워드
- 제목
- Engineered heterologous FPP synthases-mediated Z,E-FPP synthesis in E. coli
- 저자
- Wang, Chonglong; Zhou, Jia; Jang, Hui-Jeong; Yoon, Sang-Hwal; Kim, Jae-Yean; Lee, Seung-Goo; Choi, Eui-Sung; Kim, Seon-Won
- 발행일
- 2013-07
- 유형
- Article
- 권
- 18
- 페이지
- 53 ~ 59