Flavonoids from Orostachys japonicus A. Berger Inhibit the Invasion of LnCaP Prostate Carcinoma Cells by Inactivating Akt and Modulating Tight Junctionsopen access
- Authors
- Shin, Dong Yeok; Lee, Won Sup; Jung, Ji Hyun; Hong, Su Hyun; Park, Cheol; Kim, Hye Jung; Kim, Gi-Young; Hwang, Hye Jin; Kim, Gon Sup; Jung, Jin-Myung; Ryu, Chung Ho; Shin, Sung Chul; Hong, Soon Chan; Choi, Yung Hyun
- Issue Date
- Sep-2013
- Publisher
- MDPI
- Keywords
- Orostachys japonicus; flavonoids; LnCaP cells; tight junctions; Akt
- Citation
- INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v.14, no.9, pp.18407 - 18420
- Indexed
- SCIE
SCOPUS
- Journal Title
- INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
- Volume
- 14
- Number
- 9
- Start Page
- 18407
- End Page
- 18420
- URI
- https://scholarworks.bwise.kr/gnu/handle/sw.gnu/20495
- DOI
- 10.3390/ijms140918407
- ISSN
- 1661-6596
- Abstract
- Tight junctions (TJs) are a mode of cell-to-cell adhesion in epithelial or endothelial cells, and serve as a physical barrier to maintenance of homeostasis in body by controlling paracellular transport. Claudins are the most important molecules of the TJs, but paradoxically these proteins are frequently over-expressed in cancers and their overexpression is implicated in the invasive potential of cancer. Hence, we investigated the effects of flavonoids extracted from Orostachys japonicus A. Berger (FEOJ) on TJs and the expression of claudins as well as cancer invasion along with in LnCaP human prostate cancer. FEOJ suppressed cancer cell motility and invasiveness at the concentrations where FEOJ did not show anti-proliferative activity. FEOJ increased transepithelial electrical resistance (TER) associated with tightening TJs, and suppressed expression of claudin proteins. Furthermore, FEOJ suppressed the activities of MMP-2 and -9 in a dose-dependent manner, which came from the activation of tissue inhibitor of metalloproteinases (TIMPs) by FEOJ. FEOJ suppressed migration and invasion by suppressing PI3K/Akt signaling pathway. Taken together, this study suggest that FEOJ suppresses cancer migration and invasion by tightening TJs through the suppression of claudin expression, and by suppressing MMPs in LnCaP human prostate cancer cells, which at least in part results from the suppression of PI3K/Akt signaling pathway.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - 농업생명과학대학 > 식품공학부 > Journal Articles
- 수의과대학 > Department of Veterinary Medicine > Journal Articles
- College of Medicine > Department of Medicine > Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.gnu.ac.kr/handle/sw.gnu/20495)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.