In Vitro Antibacterial Effect of the Combination of Galla rhois ethanol extracts and Sodium chlorate against Intramacrophage Brucella abortus

Abstract

This study investigated the antibacterial effects of GR ethanol extracts (GRE), sodium chlorate(SC) and a combination of GRE and SC (GS) on Brucella abortus (B. abortus). The antibacterial activities of GRE,SC and GS towards B. abortus were evaluated by incubating B. abortus with GRE, SC and GS. Following treatmentwith GRE, SC and GS, B. abortus survival and intracellular proliferation in macrophages were monitored. In the cellularcytotoxicity assay, GRE, SC and GS are not cytotoxic at concentrations less than 400 μg/ml, 15 mM and 0.6GS(1 of GS, GRE 1,000 μg/ml + SC 30 mM), respectively. The viability of B. abortus was markedly decreased in a dosedependentmanner in all treatment groups. In addition, B. abortus intracellular proliferation within macrophages wassignificantly reduced in cells treated with GRE (400 μg/mL), SC (15 mM) and 0.5GS (GRE 500 μg/mL + SC 15 mM)after 48 hr-incubation (GRE, p < 0.01; SC and 0.5GS, p < 0.001). Especially, in the treatment of GS, the synergisticeffect of GRE and SC treatment on B. abortus in macrophage was observed. In conclusion, GS is useful as an antibacterialcandidate against B. abortus, and can be applied in the field of meat and milk hygiene.