OPU 유래 한우 수정란 생산 및 이식

Abstract

This study was carried out to establish the system of OPU derived embryo production, management of recipientsas well as offspring production. OPU derived embryo production system was carried out of aspiration of immatureoocytes 2 times per week, total 24 times for 3 months by an ultrasonographic guided follicular aspiration system andthen produced in vitro-produced blastocysts by in vitro maturation, fertilization and culture system. This work wascollected total 13,866 oocytes, average 8.2 ± 4.5 oocytes per session and 8,170 G1 + G2 grade oocytes, average 4.8oocytes per session by 1,692 times session of total 71 donors for 4 years from 2010 to 2013. The rate of cleavageand blastocyst developmental competence were obtained 11,825 (85.3%) and 5,032 (36.3%) that was 7.0 ± 3.8 cleavedembryos and 3.0 ± 2.5 blastocysts per session. OPU derived embryo transfer were taken place in 2, 4, 6 and 7 localgovernments at 2010, 2011, 2012 and 2013 for 4 years and pregnancy rate were obtained 41.2, 43.9, 46.5 and 49.7%in each years. It means that pregnancy rate was continuously improved according of every year for 4 years. Pregnancyrate was significantly different according to individual local government in which was 62.7% in B, but 24.2% in Fat 2012. Paternity identification was carried out total 26 offspring in C local government of 2012 and then confirmed100% agreement of its analysis. In conclusion, the results obtained the possibility of mass production of elite cowembryos as well as offspring by OPU derived embryo production system, of which could be decreased the requiredtime of genetic improvement.