콩 종자의 Raffinose 및 Stachyose 함량에 대한 유전자형과 환경의 영향

Abstract

Soybean (Glycine max (L.) Merr.) is an importantcrop for protein, oil, carbohydrates, isoflavones, and manyother nutrients to humans and animals. But, antinutritionalfactors in the raw mature soybean are exist. Raffinose andstachyose are main antinutritional factors in soybean seed. Both raffinose and stachyose are carbohydrates, belongingto the raffinose family of oligosaccharides (RFOs). RFOsare not readily digested in humans and cause flatulence ordiarrhea. The objective of this research is to obtain theinformation on raffinose and stachyose content according togenotype and environment. A total of twenty two soybeangenotypes (11 cultivars, 3 germplasms and 8 breeding lines)were selected. Each genotype was grown in the field fortwo years with two replications and harvested in bulk atnatural maturity for two years. Content of raffinose andstachyose was detected by HPLC. The raffinose content(g/kg) of 22 genotypes was 2.68±0.21 - 5.87±2.43 in year1 and was 3.24±0.37 - 9.05±0.16 in year 2. The stachyosecontent (g/kg) was 4.23±0.98 - 27.68±9.90 at year 1 and was5.11±1.09 - 25.32±0.35 in year 2. Genotype and environment havehighly significant effects on raffinose and stachyose content. Three genotypes (Da-7, 116-13, and RS-78) have low stachyosecontent at 5% significant level in two years. Apositive correlation (R2=0.1985*) between raffinose and stachyosewas observed in year 2. These informations are valuable insoybean genetics and breeding program related with raffinoseand stachyose content.