정제봉독(PBV)과 분리정제봉독(IPBV), 봉독아민(BVA)분획시료에 대한 화장소재로서의 효능비교

Abstract

The skin care efficacies for three fractions of bee venom as cosmetic raw materials were evaluated. The purified bee venom(PBV) was obtained by removing non-soluble species and bacteria through 0.2 μm syringe filter from the collected bee venom with Chung Jin Biotech’s electrical shock collector. PBV was treated to isolated bee venom(IPBV) with ultrafiltration of 10 KDa membrane and bee venom amines(BVA) with ultra filtration of 1 KDa membrane. The properties of these bee venoms were measured and evaluated by DPPH radical scavenging assay, tyrosinase inhibitory effect, elastase inhibitory effect, and MTT assay for cytotoxicity. As a result of the experiment, DPPH radical scavenging abilities were confirmed that 0.32 ±0.04 mg/mL for PBV, 0.53 ± 0.10 mg/mL for IPBV, and 0.09 ± 0.01 mg/mL for BVA as the half maximal inhibitory concentration, and tyrosinase inhibitory effect as IC50 were confirm each sample concentration of PBV, IPBV and BVA to 2.93 ± 0.01 mg/mL, 4.65± 0.012 mg/mL and 2.19± 0.01 mg/mL. The result of Elastase inhibitory effect were observed 2.09 ±0.031 mg/mL to PBV, 1.62 ±0.012 mg/mL to IPBV and none to BVA as value of IC50. It is shown that the cell viability concentration is 90% above 0.4 ±0.01 μg/mL in PBV, above 0.64±0.012 μg/mL in IPBV and 110 ± 0.01 μg/mL in BVA, respectively. There were almost same tendency between PBV and IPBV, while BVA showed better antioxidant activity and whitening effect with no citotoxicity.