Proteomic Analysis of Testicular Ischemia-Reperfusion Injury in Ratsopen access
- Authors
- Ouh, In-Ohk; Seo, Min-Goo; Shah, Fawad-Ali; Gim, Sang-Ah; Koh, Phil-Ok
- Issue Date
- Mar-2014
- Publisher
- JAPAN SOC VET SCI
- Keywords
- ischemia-reperfusion injury; proteomics; rat; testicular torsion-detorsion
- Citation
- JOURNAL OF VETERINARY MEDICAL SCIENCE, v.76, no.3, pp.313 - 321
- Indexed
- SCIE
SCOPUS
- Journal Title
- JOURNAL OF VETERINARY MEDICAL SCIENCE
- Volume
- 76
- Number
- 3
- Start Page
- 313
- End Page
- 321
- URI
- https://scholarworks.bwise.kr/gnu/handle/sw.gnu/19141
- DOI
- 10.1292/jvms.13-0248
- ISSN
- 0916-7250
- Abstract
- Testicular torsion is a urological emergency that leads to serious testicular damage and male infertility. We performed this study to identify specific proteins that are differentially expressed in response to testicular torsion and detorsion-induced ischemia-reperfusion (I-R) injury. Adult male rats were divided into two groups: a sham-operated group and a testicular I-R group. Testicular torsion was induced by rotating the left testis 720 degrees in a clockwise direction for 1 hr, and then, detorsion was performed for 24 hr. After this testicular tissues were collected, protein analysis was performed using two-dimensional gel electrophoresis and Western blot analyses. Testicular I-R injury resulted in serious histopathologic damage to the germinal cells in the seminiferous tubules and increased the number of TUNEL-positive cells in testicular tissue. Specific protein spots with a greater than 2.5-fold change in intensity between the sham-operated and testicular I-R groups were identified by mass spectrometry. Among these proteins, levels of peroxiredoxin 6, thioredoxin, heterogeneous nuclear ribonucleoproteins, ubiquitin carboxyl terminal hydrolase isozyme L5 and zinc finger AN1-type domain 3 were decreased in the testicular I-R group compared to the sham-operated group. Moreover, Western blot analysis clearly showed the decrease of these proteins in the testicular I-R group. These proteins have spermatogenesis and anti-oxidative functions. These findings suggest that testicular I-R results in cell death due to altered expression of several proteins with spermatogenesis and anti-oxidation functions.
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