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Proteinase K-containing Lipid Nanoparticles for Therapeutic Delivery of siRNA LOR-1284
- Issue Date
- Jul-2014
- Publisher
- INT INST ANTICANCER RESEARCH
- Keywords
- Lipid nanoparticle; siRNA; proteinase K; cancer; ribonucleotide reductase
- Citation
- ANTICANCER RESEARCH, v.34, no.7, pp 3531 - 3535
- Pages
- 5
- Indexed
- SCI
SCIE
SCOPUS
- Journal Title
- ANTICANCER RESEARCH
- Volume
- 34
- Number
- 7
- Start Page
- 3531
- End Page
- 3535
- ISSN
- 0250-7005
1791-7530
- Abstract
- Background: The objective of the present study was to develop an efficient delivery vehicle for siRNA LOR-1284 through incorporation of proteinase K (PrK) as a means of preventing siRNA degradation by serum nucleases. Lipid nanoparticle-PrK-siRNA (LN-PrK-siRNA) complexes were synthesized and characterized. Materials and Methods: siRNA complexed with PrK and liposomes composed of dimethyldioctadecyl ammonium bromide/cholesterol/Tween 80 (60:35:5 molar ratio) were investigated for down-regulation of R2 mRNA activity in KB human carcinoma cells. Results: Treatment with LN-PrK-siRNA (30:0.3:1 molar ratio) significantly reduced levels of R2 mRNA compared to siRNA-liposomes without PrK in serum-containing medium. LN-PrK-siRNA complexes showed increased stability in serum and reduced toxicity in KB cells relative to LN-siRNA complexes. Conclusion: LN-PrK-siRNA complexes are promising delivery vehicles for siRNA.
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