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Properties of a Novel Glutamate Decarboxylase (GAD) from Levilactobacillus brevis B737 Isolated from Cabbage Kimchiopen accessProperties of a Novel Glutamate Decarboxylase (GAD) from Levilactobacillus brevis B737 Isolated from Cabbage Kimchi

Other Titles
Properties of a Novel Glutamate Decarboxylase (GAD) from Levilactobacillus brevis B737 Isolated from Cabbage Kimchi
Authors
Kim Tae JinKim Min JaeKim Bong SinYoo Ji YeonKang Yun Ji김정환
Issue Date
28-Sep-2022
Publisher
한국미생물·생명공학회
Keywords
GABA; Levilactobacillus brevis; glutamate decarboxylase; cabbage kimchi
Citation
한국미생물·생명공학회지, v.50, no.3, pp 319 - 327
Pages
9
Indexed
SCOPUS
Journal Title
한국미생물·생명공학회지
Volume
50
Number
3
Start Page
319
End Page
327
URI
https://scholarworks.gnu.ac.kr/handle/sw.gnu/1871
DOI
10.48022/mbl.2206.06006
ISSN
1598-642X
Abstract
γ-Aminobutyric acid (GABA) is a multi-functional compound with broad applications for food industry. GABA producing bacteria were isolated from cabbage kimchi. Among them, B737 was the best GABA producer when culture supernatants were analyzed by TLC. B737 was identified as Levilactobacillus brevis by 16S rRNA gene sequencing. Its glutamate decarboxylase (GAD) gene was cloned by PCR and the nucleotide sequence determined. B737 GAD consisting of 485 amino acids is the largest in size among GADs reported from LAB so far. gadB from L. brevis B737 was overexpressed in Escherichia. coli BL21(DE3) using pET26b(+). The recombinant GAD was purified and its size was 55 kDa by SDS-PAGE. Maximum GAD activity was observed at pH 5 and 40℃ and the activity was dependent on pyridoxal 5'-phosphate. Km and Vmax of recombinant GAD were 6.2 ± 0.06 mM and 0.34 ± 0.002 mM/min, respectively. L. brevis B737 can be used as a starter for fermented foods with high GABA contents.
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