Proteomic analysis of ageing in black gram (Vigna mungo L.) seeds and its relation to seed viability
- Authors
- Sathish, S.; Ahamed, R.; Senthil, N.; Arulkumar, N.; Park, H.S.; Kalaiselvi, S.; Umarani, R.; Raveendran, M.; Bhaskaran, M.; Kim, G.S.
- Issue Date
- 2015
- Publisher
- Southern Cross Publishing
- Keywords
- Actin; Black gram; Cell structure; PaO; Proteomics; Seed ageing; Seed viability; VARS; Vigour
- Citation
- Plant OMICS, v.8, no.3, pp.201 - 211
- Indexed
- SCOPUS
- Journal Title
- Plant OMICS
- Volume
- 8
- Number
- 3
- Start Page
- 201
- End Page
- 211
- URI
- https://scholarworks.bwise.kr/gnu/handle/sw.gnu/18388
- ISSN
- 1836-0661
- Abstract
- The Indian minimum seed certification standards recommend that black gram seeds should have a minimum germination of at least 75% in order to pass the germination standard. The aim of this study is to investigate, using 2D-PAGE analysis, the possible proteome changes in black gram seeds when their germination rate falls below 75% and thereby, identify proteins that correlate with loss of seed viability during seed ageing. Firstly, in order to obtain seed samples with germination rate less than 75%, for 2D-PAGE analysis, we analyzed the proteomic (using SDS-PAGE) and physiological changes in seeds, which were artificially aged for 1 to 10 days at 98 ± 2% relative humidity and 40 ± 1° C temperature. The results revealed the major physiological and protein changes occurs from sixth day onwards and germination rate falls below 75% at sixth day, suggesting that 2D-PAGE seed samples can be obtained from 6-day-artificially aged seeds. Secondly, we carried out 2D-PAGE analysis using fresh and 6-day-artificially aged seeds and characterized 16 differentially expressed proteins in aged seeds. These proteins were identified using MALDI-TOF-MS and classified into 8 functional groups. Out of 16 proteins, 4 were up-regulated and 12 were down-regulated, indicating that these proteins play an important role in seed viability. Seven down-regulated proteins were functionally related to cell structure, transporters, metabolism and transcription, and one up-regulated protein was related to defense. In summary, this study has identified proteins that correlate with seed deterioration and loss of viability during ageing in black gram seeds.
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