베타글루칸 함량이 높은 큰느타리버섯 선발을 위한 SCAR marker 개발

Abstract

In this study, SCAR marker that differentiates Pleurotus eryngii strains with higher β-glucan from control strain wasdeveloped. Genomic DNAs of 9 control strains of Pleurotus eryngii and 9 Pleurotus eryngii strains with higher β-glucan wereanalyzed by bulked segregant analysis (BSA) using randomly amplified polymorphic DNA (RAPD). One-hundred twenty RAPDprimers were screened on bulked DNA samples and a unique DNA fragment with the size of 91 bp was yielded by OP-R03primer from the Pleurotus eryngii strains with higher β-glucan. A sequence characterized amplified region (SCAR) marker,designated as OP-R03-1-F and OP-R03-1-R, was designed on the basis of the determined sequence. The PCR analysis with theOP-R03-1 primer showed that this SCAR marker can clearly distinguish the Pleurotus eryngii strains with higher β-glucan from thecontrol strains.