Comparison between Immunization Routes of Live Attenuated Salmonella Typhimurium Strains Expressing BCSP31, Omp3b, and SOD of Brucella abortus in Murine Model

Abstract

Live, attenuated Salmonella Typhimurium vaccine candidate expressing BCSP31, Omp3b, and SOD proteins of Brucella abortus was constructed. Thirty BALB/c mice were divided equally into three groups, Group A, were intraperitoneally (IP) inoculated with 100 mu l of approximately 1.2 x 10(6) colony-forming units (CFUs)/ml of the Salmonella containing vector only in 100 mu l as a control. And groups B and C mice were orally and IP immunized with approximately 1.2 x 10(9) CFU/ml of the mixture of three delivery strains in 10 ill and IP immunized with approximately 1.2 x 10(6) CFU/ml of the mixture in 100 mu l, respectively. The serum IgG, TNF-alpha and IFN-gamma concentrations in groups B (except Omp3b) and C were significantly higher than those in group A. Following challenge with B. abortus strain 544; challenge strain was detected <10(3) CFU from the spleen of all mice of group C. These results suggest that IP immunization with the mixture of the vaccine candidate can induce immune responses, and can effectively protect mice against brucellosis.