Development of Porous Beads to Provide Regulated BMP-2 Stimulation for Varying Durations: In Vitro and In Vivo Studies for Bone Regeneration
- Authors
- Kim, Ho Yong; Lee, Jin Ho; Yun, Jeong-Won; Park, Jin-Ho; Park, Bong-Wook; Rho, Gyu-Jin; Jang, Si-Jung; Park, Ji-Sung; Lee, Hee-Chun; Yoon, Young Min; Hwang, Tae Sung; Lee, Dong Hoon; Byun, June-Ho; Oh, Se Heang
- Issue Date
- May-2016
- Publisher
- AMER CHEMICAL SOC
- Citation
- BIOMACROMOLECULES, v.17, no.5, pp.1633 - 1642
- Indexed
- SCIE
SCOPUS
- Journal Title
- BIOMACROMOLECULES
- Volume
- 17
- Number
- 5
- Start Page
- 1633
- End Page
- 1642
- URI
- https://scholarworks.bwise.kr/gnu/handle/sw.gnu/15513
- DOI
- 10.1021/acs.biomac.6b00009
- ISSN
- 1525-7797
- Abstract
- It is commonly accepted that the sustained release of bone morphogenetic protein-2 (BMP-2) can enhance bone regeneration and minimize its safety issues. However, little is known regarding the appropriate duration of BMP-2 stimulation for sufficient osteogenic differentiation and new bone formation because of the short half-life of BMP-2 in the physiological environment and the lack of a well-defined delivery matrix that can regulate the release period of BMP-2. In this study, we prepared porous poly(lactic-co-glycolic acid) (PLGA) beads with different surface pore sizes that can regulate the release period of BMP-2 (i.e., 7, 17, and 30 days) while providing the BMP-2 concentration required for bone regeneration. Our findings in both in vitro cell culture and in vivo animal studies using these BMP-2-loaded beads demonstrate that release of BMP-2 within 7 days affects only the initial differentiation of human periosteum-derived cells (hPDCs) and does not significantly enhance their subsequent differentiation into mature functional cells. However, extending the duration of BMP-2 stimulation over 17 days can provide a suitable environment for osteogenic differentiation of hPDCs and new bone formation.
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Collections - College of Medicine > Department of Medicine > Journal Articles
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