Antiamnesic Effect of Broccoli (Brassica oleracea var. italica) Leaves on Amyloid Beta (A beta)(1-42)-Induced Learning and Memory Impairment

Abstract

To examine the antiamnesic effects of broccoli (Brassica oleracea var. italica) leaves, we performed. in vitro and in vivo tests on amyloid beta (A beta)-induced neurotoxicity. The chloroform fraction from broccoli leaves (CBL) showed a remarkable neuronal cell-protective effect and an inhibition against acetylcholinesterase (AChE). The ameliorating-effect of CBL on A beta(1-42)-induced learning and memory impairment was evaluated by Y-maze, passive avoidance, and Morris water maze tests. The results indicated improving cognitive function in the CBL group. After the behavioral tests, antioxidant effects were detected by superoxide dismutase (SOD), oxidized glutathione (GSH)/total GSH, and malondialdehyde (MDA) assays, and inhibition against AChE was also presented in the brain. Finally, oxo-dihydroxy-octadecenoic acid (oxo-DHODE) and trihydroxy-octadecenoic acid (THODE) as main compounds were identified by quadrupole time-of-flight ultraperformance liquid chromatography (Q-TOF UPLC-MS) analysis. Therefore, our studies suggest that CBL could be used as a natural resource for ameliorating A beta(1-42)-induced learning and memory impairment.