Advanced Whole-cell Conversion for D-allulose Production Using an Engineered Corynebacterium glutamicum
- Jeong, Seong-Hee; Kwon, Moonhyuk; Kim, Seon-Won
- Issue Date
- KOREAN SOC BIOTECHNOLOGY & BIOENGINEERING
- Corynebacterium glutamicum; whole-cell conversion; D-allulose; D-allulose 3-epimerase; high temperature process
- BIOTECHNOLOGY AND BIOPROCESS ENGINEERING, v.27, no.2, pp.276 - 285
- Journal Title
- BIOTECHNOLOGY AND BIOPROCESS ENGINEERING
- Start Page
- End Page
- D-allulose has received considerable attention as an alternative functional sugar for its zero caloric value with 70% relative sweetness compared to D-sucrose. Despite its strong potential as an alternative sweetener, recent industrial productions rely on a high-cost enzymatic method. Here, we advanced whole-cell conversion at high temperatures using Corynebacterium glutamicum expressing D-allulose 3-epimerase (DAE). By varying the reaction temperature from 25 degrees C to 70 degrees C, D-allulose conversion could reach the reaction equilibrium at high temperatures. The C. glutamicum showed superior reusability of cells at 60 degrees C compared to Escherichia coli. We simplified the cell growth media and whole-cell conversion reaction solution. Clostridium hylemonae DAE (ChDAE) showed the highest thermostability and reusability among various DAE candidates. Finally, the ChDAE expression under the synthetic 2X-cT-T5 promoter could reduce the reaction time by 25%. Our result showed that 120 g/L of D-allulose can be produced from 400 g/L of D-fructose by reusable whole-cell conversion at 55 degrees C in 1.5 h. This study can be highly applicable in industrial economic production.
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- 자연과학대학 > Division of Life Sciences > Journal Articles
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