Detailed Information

Cited 5 time in webofscience Cited 5 time in scopus
Metadata Downloads

Extracellular vesicles improve embryo cryotolerance by maintaining the tight junction integrity during blastocoel re-expansionopen access

Authors
Sidrat, TabindaKhan, Abdul AzizJoo, Myeong-DonXu, LianguangEl-Sheikh, MarwaKo, Jong-HyukKong, Il-Keun
Issue Date
Apr-2022
Publisher
BIOSCIENTIFICA LTD
Citation
REPRODUCTION, v.163, no.4, pp.219 - 232
Indexed
SCIE
SCOPUS
Journal Title
REPRODUCTION
Volume
163
Number
4
Start Page
219
End Page
232
URI
https://scholarworks.bwise.kr/gnu/handle/sw.gnu/1458
DOI
10.1530/REP-21-0320
ISSN
1470-1626
Abstract
Cryopreservation is a process in which the intact living cells, tissues, or embryos are preserved at subzero temperatures for preservation. The cryopreservation process highly impacts the survival and quality of the in vitro-produced (IVP) embryos. Some studies have highlighted the use of oviduct extracellular vesicles (EVs) to improve the cryotolerance of IVP embryos but the mechanism has not been well studied. The present study unravels the role of in vitro cultured bovine oviduct epithelial cells-derived EVs in improving the re-expansion and hatching potential of thawed blastocysts (Us). The comparison of cryotolerance between synthetic oviduct fluid (SOF) and SOF+ EVs-supplemented day-7 cryopreserved BLs revealed that the embryo's ability to re-expand critically depends on the intact paracellular sealing which facilitates increased fluid accumulation during cavity expansion after shrinkage. Our results demonstrated that BLs cultured in the SOF+ EVs group had remarkably higher re-expansion (67.5 +/- 4.2%) and hatching rate (84.8 +/- 1.4%) compared to the SOF group (53.4 +/- 3.4% and 63.9 +/- 0.9%, respectively). Interestingly, EVssupplemented BLs exhibited greater influence on the expression of core genes involved in trophectoderm (FE) maintenance, formation of tight junction (1)) assembly, H2O channel proteins (aquaporins), and Na+/K+ ATPase alpha 1. The EVs improved the fluid flux and allowed the transport of H2O into an actively re-expanded cavity in EVs-cultured cryo-survived BLs relative to control BLs. Our findings explored the function of EVs in restoring the TE integrity, improved the cell junctional contacts and H2O movement which helps the blastocoel re-expansion after thawing the cryopreserved BLs.
Files in This Item
There are no files associated with this item.
Appears in
Collections
ETC > Journal Articles

qrcode

Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.

Related Researcher

Researcher Kong, Il Keun photo

Kong, Il Keun
대학원 (응용생명과학부)
Read more

Altmetrics

Total Views & Downloads

BROWSE