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Specific Detection of Acanthamoeba species using Polyclonal Peptide Antibody Targeting the Periplasmic Binding Protein of A. castellaniiopen access

Authors
Kim, Min-JeongQuan, Fu-ShiKong, Hyun-HeeKim, Jong-HyunMoon, Eun-Kyung
Issue Date
Apr-2022
Publisher
KOREAN SOC PARASITOLOGY, SEOUL NATL UNIV COLL MEDI
Keywords
Acanthamoeba keratitis; periplasmic binding protein; peptide antibody; species specificity
Citation
KOREAN JOURNAL OF PARASITOLOGY, v.60, no.2, pp.143 - 147
Indexed
SCIE
SCOPUS
KCI
Journal Title
KOREAN JOURNAL OF PARASITOLOGY
Volume
60
Number
2
Start Page
143
End Page
147
URI
https://scholarworks.bwise.kr/gnu/handle/sw.gnu/1440
DOI
10.3347/kjp.2022.60.2.143
ISSN
0023-4001
Abstract
Acanthamoeba keratitis (AK) is a rare ocular disease, but it is a painful and sight-threatening infectious disease. Early diagnosis and adequate treatment are necessary to prevent serious complications. While AK is frequently diagnosis via several PCR assays or Acanthamoeba-specific antibodies, a more specific and effective diagnostic method is required. This study described the production of a polyclonal peptide antibody against the periplasmic binding protein (PBP) of A. castellanii and investigated its diagnostic potential. Western blot analysis showed that the PBP antibody specifically reacted with the cell lysates of A. castellanii. However, the PBP antibody did not interact with human corneal epithelial (HCE) cells and the other 3 major causative agents of keratitis. Immunocytochemistry (ICC) results revealed the specific detection of A. castellanii trophozoites and cysts by PBP antibodies when A. castellanii were co-cultured with HCE cells. PBP antibody specificity was further confirmed by co-culture of A. castellanii trophozoites with F. solani, S. aureus, and P. aeruginosa via ICC. The PBP antibody specifically reacted with the trophozoites and cysts of A. polyphaga, A. hatchetti, A. culbertsoni, A. royreba, and A. healyi, thus demonstrated its genus-specific nature. These results showed that the PBP polyclonal peptide antibody of A. castellanii could specifically detect several species of Acanthamoeba, contributing to the development of an effective antibody-based AK diagnostics.
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