Nocodazole treatment interrupted Brucella abortus invasion in RAW 264.7 cells, and successfully attenuated splenic proliferation with enhanced inflammatory response in mice
- Authors
- Reyes, Alisha Wehdnesday Bernardo; Huynh Tan Hop; Arayan, Lauren Togonon; Tran Xuan Ngoc Huy; Min, Wongi; Lee, Hu Jang; Chang, Hong Hee; Kim, Suk
- Issue Date
- Feb-2017
- Publisher
- ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
- Keywords
- B. abortus; Nocodazole; Macrophages; F-actin; MAPKs; Cytokine
- Citation
- MICROBIAL PATHOGENESIS, v.103, pp.87 - 93
- Indexed
- SCIE
SCOPUS
- Journal Title
- MICROBIAL PATHOGENESIS
- Volume
- 103
- Start Page
- 87
- End Page
- 93
- URI
- https://scholarworks.bwise.kr/gnu/handle/sw.gnu/13915
- DOI
- 10.1016/j.micpath.2016.11.028
- ISSN
- 0882-4010
- Abstract
- Brucellosis is one of the most important and widespread zoonosis worldwide responsible for serious economic losses and considerable public health burden. In this study, we investigated the modulatory effect of a microtubule-inhibitor, nocodazole, on B. abortus infection in murine macrophages and in a mouse model. Nocodazole activated macrophages and directly inhibited the growth of Brucella in a dose dependent manner. Nocodazole increased adhesion but reduced invasion and intracellular growth of Brucella in macrophages although it did not affect co-localization of Brucella with LAMP-1. In addition, nocodazole negatively affected actin polymerization, and weakly activated ERK and p38 alpha but significantly activated JNK in non-infected cells. After subsequent infection, nocodazole weakly inhibited activation of ERK and p38 alpha. For the in vivo tests, nocodazole-treated mice displayed elevated levels of IFN-gamma, MCP-1 and IL-10 while Brucella-infected nocodazole-treated mice showed high levels of TNF, IFN-gamma, MCP-1, IL-10 and IL-6 as compared to controls. Furthermore, nocodazole treatment reduced inflammation and Brucella proliferation in the spleens of mice. These findings highlight the potential use of nocodazole for the control of brucellosis although further investigations are encouraged to validate its therapeutic use in animal hosts. (C) 2016 Elsevier Ltd. All rights reserved.
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Collections - 농업생명과학대학 > 축산과학부 > Journal Articles
- 수의과대학 > Department of Veterinary Medicine > Journal Articles
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