Improvement of in vitro-produced bovine embryo treated with coagulansin-A under heat-stressed conditionopen access
- Authors
- Khan, Imran; Lee, Kyeong-Lim; Xu, Lianguang; Mesalam, Ayman; Chowdhury, M. M. R.; Joo, Myeong-Don; Ihsan-ul-Haq; Mirza, Bushra; Kong, Il-Keun
- Issue Date
- Apr-2017
- Publisher
- BIOSCIENTIFICA LTD
- Citation
- REPRODUCTION, v.153, no.4, pp.421 - 431
- Indexed
- SCIE
SCOPUS
- Journal Title
- REPRODUCTION
- Volume
- 153
- Number
- 4
- Start Page
- 421
- End Page
- 431
- URI
- https://scholarworks.bwise.kr/gnu/handle/sw.gnu/13794
- DOI
- 10.1530/REP-16-0530
- ISSN
- 1470-1626
- Abstract
- Heat stress has large effects on reproduction including conception rate in cattle. In this study, we examined the effects of coagulansin-A (coa-A), a steroidal lactone, on acquired thermo tolerance during in vitro production of bovine embryos. Oocytes were incubated in in vitro maturation (IVM) media with or without coa-A at two different temperatures, 40.5 degrees C and 42 degrees C, for 20 h. The treatment of coa-A significantly improved blastocyst development only at 40.5 degrees C (P < 0.05). Interestingly, immunofluorescence analysis demonstrated that coa-A induced heat shock protein 70 (HSP70) and phosphatidylinositol-3-kinase (PI3K), but significantly attenuated nuclear factor kappa B (NF-kappa B) and cyclooxygenase-2 (COX2). To determine the expression patterns of related genes at the transcription level, qRT-PCR was performed. Expression of HSP70 and PI3K was elevated, whereas expression of NF-kappa B, COX2 and inducible nitric oxide synthase (iNOS) was significantly (P < 0.05) downregulated in the coa-A-treated group compared with the control group. Moreover, pro-apoptotic genes were downregulated, and antiapoptic genes were upregulated in the coa-A group. We also counted the total cell number and apoptotic nuclei at the blastocyst and found that more cell numbers (143.1 +/- 1.5) and less apoptotic damages (6.4 +/- 0.5) in the coa-A treatment group comparing to control group (131.4 +/- 2.0 and 10.8 +/- 0.5), indicating the enhanced embryo quality. In conclusion, our results demonstrate that the coa-A not only improved the blastocyst development in vitro but also increased their resistance to heat stress condition through induction of HSP70/PI3K.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - ETC > Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.gnu.ac.kr/handle/sw.gnu/13794)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.