Improvement of in vitro-produced bovine embryo treated with coagulansin-A under heat-stressed condition
DC Field | Value | Language |
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dc.contributor.author | Khan, Imran | - |
dc.contributor.author | Lee, Kyeong-Lim | - |
dc.contributor.author | Xu, Lianguang | - |
dc.contributor.author | Mesalam, Ayman | - |
dc.contributor.author | Chowdhury, M. M. R. | - |
dc.contributor.author | Joo, Myeong-Don | - |
dc.contributor.author | Ihsan-ul-Haq | - |
dc.contributor.author | Mirza, Bushra | - |
dc.contributor.author | Kong, Il-Keun | - |
dc.date.accessioned | 2022-12-26T18:48:28Z | - |
dc.date.available | 2022-12-26T18:48:28Z | - |
dc.date.created | 2022-12-13 | - |
dc.date.issued | 2017-04 | - |
dc.identifier.issn | 1470-1626 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gnu/handle/sw.gnu/13794 | - |
dc.description.abstract | Heat stress has large effects on reproduction including conception rate in cattle. In this study, we examined the effects of coagulansin-A (coa-A), a steroidal lactone, on acquired thermo tolerance during in vitro production of bovine embryos. Oocytes were incubated in in vitro maturation (IVM) media with or without coa-A at two different temperatures, 40.5 degrees C and 42 degrees C, for 20 h. The treatment of coa-A significantly improved blastocyst development only at 40.5 degrees C (P < 0.05). Interestingly, immunofluorescence analysis demonstrated that coa-A induced heat shock protein 70 (HSP70) and phosphatidylinositol-3-kinase (PI3K), but significantly attenuated nuclear factor kappa B (NF-kappa B) and cyclooxygenase-2 (COX2). To determine the expression patterns of related genes at the transcription level, qRT-PCR was performed. Expression of HSP70 and PI3K was elevated, whereas expression of NF-kappa B, COX2 and inducible nitric oxide synthase (iNOS) was significantly (P < 0.05) downregulated in the coa-A-treated group compared with the control group. Moreover, pro-apoptotic genes were downregulated, and antiapoptic genes were upregulated in the coa-A group. We also counted the total cell number and apoptotic nuclei at the blastocyst and found that more cell numbers (143.1 +/- 1.5) and less apoptotic damages (6.4 +/- 0.5) in the coa-A treatment group comparing to control group (131.4 +/- 2.0 and 10.8 +/- 0.5), indicating the enhanced embryo quality. In conclusion, our results demonstrate that the coa-A not only improved the blastocyst development in vitro but also increased their resistance to heat stress condition through induction of HSP70/PI3K. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | BIOSCIENTIFICA LTD | - |
dc.subject | NF-KAPPA-B | - |
dc.subject | BUBALUS-BUBALIS EMBRYOS | - |
dc.subject | DEVELOPMENTAL COMPETENCE | - |
dc.subject | MOLECULAR CHAPERONES | - |
dc.subject | INDUCED APOPTOSIS | - |
dc.subject | OXIDATIVE STRESS | - |
dc.subject | GENE-EXPRESSION | - |
dc.subject | SHOCK PROTEINS | - |
dc.subject | DAIRY-COWS | - |
dc.subject | CELL-DEATH | - |
dc.title | Improvement of in vitro-produced bovine embryo treated with coagulansin-A under heat-stressed condition | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kong, Il-Keun | - |
dc.identifier.doi | 10.1530/REP-16-0530 | - |
dc.identifier.scopusid | 2-s2.0-85014524411 | - |
dc.identifier.wosid | 000395830100008 | - |
dc.identifier.bibliographicCitation | REPRODUCTION, v.153, no.4, pp.421 - 431 | - |
dc.relation.isPartOf | REPRODUCTION | - |
dc.citation.title | REPRODUCTION | - |
dc.citation.volume | 153 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 421 | - |
dc.citation.endPage | 431 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Developmental Biology | - |
dc.relation.journalResearchArea | Reproductive Biology | - |
dc.relation.journalWebOfScienceCategory | Developmental Biology | - |
dc.relation.journalWebOfScienceCategory | Reproductive Biology | - |
dc.subject.keywordPlus | NF-KAPPA-B | - |
dc.subject.keywordPlus | BUBALUS-BUBALIS EMBRYOS | - |
dc.subject.keywordPlus | DEVELOPMENTAL COMPETENCE | - |
dc.subject.keywordPlus | MOLECULAR CHAPERONES | - |
dc.subject.keywordPlus | INDUCED APOPTOSIS | - |
dc.subject.keywordPlus | OXIDATIVE STRESS | - |
dc.subject.keywordPlus | GENE-EXPRESSION | - |
dc.subject.keywordPlus | SHOCK PROTEINS | - |
dc.subject.keywordPlus | DAIRY-COWS | - |
dc.subject.keywordPlus | CELL-DEATH | - |
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