Inhibitory Effect of Mitoxantrone on Collagen Synthesis in Dermal Fibroblastsopen access
- Authors
- Kim, Kyung-Il; Kwon, Chang-Il; Lee, Jeung-Hoon; Kim, Chang-Deok; Yoon, Tae-Jin
- Issue Date
- Jun-2022
- Publisher
- 대한피부과학회
- Keywords
- Collagen; Fibroblasts; LARP6; Mitoxantrone; SMAD
- Citation
- Annals of Dermatology, v.34, no.3, pp 206 - 211
- Pages
- 6
- Indexed
- SCIE
SCOPUS
KCI
- Journal Title
- Annals of Dermatology
- Volume
- 34
- Number
- 3
- Start Page
- 206
- End Page
- 211
- URI
- https://scholarworks.gnu.ac.kr/handle/sw.gnu/1204
- DOI
- 10.5021/ad.2022.34.3.206
- ISSN
- 1013-9087
2005-3894
- Abstract
- Background: Fibroblasts produce collagen molecules that support the structure of the skin. The decrease and hypersynthesis of collagen causes skin problems such as skin atrophy, wrinkles and scars. Objective: The purpose of this study is to investigate the mechanism of mitoxantrone on collagen synthesis in fibroblasts. Methods: Cultured fibroblasts were treated with mitoxantrone, and then collagen synthesis was confirmed by reverse transcription-polymerase chain reaction and Western blot. Results: Mitoxantrone inhibited the expression of type I collagen in fibroblasts at both the mRNA and protein levels. In the collagen gel contraction assay, mitoxantrone significantly inhibited gel contraction compared to the control group. Mitoxantrone inhibited transforming growth factor (TGF)-(3-induced phosphorylation of SMAD3. Finally, mitoxantrone inhibited the expression of LARP6, an RNA-binding protein that regulates collagen mRNA stability. Conclusion: These results suggest that mitoxantrone reduces collagen synthesis by inhibiting TGF-(3/SMAD signaling and LARP6 expression in fibroblasts, which can be developed as a therapeutic agent for diseases caused by collagen hypersynthesis.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - College of Medicine > Department of Medicine > Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.