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Cited 30 time in webofscience Cited 34 time in scopus
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Scutellarein Inhibits LPS-Induced Inflammation through NF-κB/MAPKs Signaling Pathway in RAW264.7 Cellsopen access

Authors
Park, M.Y.Ha, S.E.Kim, H.H.Bhosale, P.B.Abusaliya, A.Jeong, S.H.Park, J.-S.Heo, J.D.Kim, G.S.
Issue Date
Jun-2022
Publisher
MDPI
Keywords
LPS-induced inflammation; MAPK; NF-кB; scutellarein (SCU)
Citation
Molecules, v.27, no.12
Indexed
SCIE
SCOPUS
Journal Title
Molecules
Volume
27
Number
12
URI
https://scholarworks.gnu.ac.kr/handle/sw.gnu/1186
DOI
10.3390/molecules27123782
ISSN
1420-3049
1420-3049
Abstract
Inflammation is a severe topic in the immune system and play a role as pro-inflammatory mediators. In response to such inflammatory substances, immune cells release cytokines such as tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β). Lipopolysaccharide (LPS) is known as an endotoxin in the outer membrane of Gram-negative bacteria, and it catalyzes inflammation by stimulating the secretion of inflammatory-mediated cytokines such as cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) by stimulated immune cells. Among the pathways involved in inflammation, nuclear factor kappa (NF-кB) and mitogen-activated protein kinases (MAPKs) are important. NF-kB is a diploid composed of p65 and IkBα and stimulates the pro-gene. MAPKs is a family consisting of the extracellular signal-regulated kinase (ERK), c-Jun NH2-terminal kinase (JNK), and p38, JNK and p38 play a role as proinflammatory mediators. Thus, we aim to determine the scutellarein (SCU) effect on LPS stimulated RAW264.7 cells. Furthermore, since scutellarein has been shown to inhibit the SARS coronavirus helicase and has been used in Chinese medicine to treat inflammatory disorders like COVID-19, it would be required to examine scutellarein’s anti-inflammatory mechanism. We identified inflammation-inducing substances using western blot with RAW264.7 cells and SCU. And we discovered that was reduced by treatment with SCU in p-p65 and p-IκBα. Also, we found that p-JNK and p-ERK were also decreased but there was no effect in p-p38. In addition, we have confirmed that the iNOS was also decreased after treatment but there is no change in the expression of COX-2. Therefore, this study shows that SCU can be used as a compound to treat inflammation. ? 2022 by the authors. Licensee MDPI, Basel, Switzerland.
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