Interleukin 10 suppresses lysosome-mediated killing of Brucella abortus in cultured macrophagesopen access
- Authors
- Hop, Huynh Tan; Reyes, Alisha Wehdnesday Bernardo; Huy, Tran Xuan Ngoc; Arayan, Lauren Togonon; Min, WonGi; Lee, Hu Jang; Rhee, Man Hee; Chang, Hong Hee; Kim, Suk
- Issue Date
- 2-Mar-2018
- Publisher
- AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
- Citation
- JOURNAL OF BIOLOGICAL CHEMISTRY, v.293, no.9, pp.3134 - 3144
- Indexed
- SCIE
SCOPUS
- Journal Title
- JOURNAL OF BIOLOGICAL CHEMISTRY
- Volume
- 293
- Number
- 9
- Start Page
- 3134
- End Page
- 3144
- URI
- https://scholarworks.bwise.kr/gnu/handle/sw.gnu/11820
- DOI
- 10.1074/jbc.M117.805556
- ISSN
- 0021-9258
- Abstract
- Brucella abortus is a Gram-negative zoonotic pathogen for which there is no 100% effective vaccine. Phagosomes in B. abortus infected cells fail to mature, allowing the pathogen to survive and proliferate. Interleukin 10 (IL10) promotes B. abortus persistence in macrophages by mechanisms that are not fully understood. In this study, we investigated the regulatory role of IL10 in the immune response to B. abortus infection. B. abortus infected macrophages were treated with either IL10 s1RNA or recombinant IL10 (rIL10), and the expression of phagolysosome- or inflammation-related genes was evaluated by qRT-PCR and Western blotting. Phagolysosome fusion was monitored by fluorescence microscopy. We found that the synthesis of several membrane-trafficking regulators and lysosomal enzymes was suppressed by IL10 during infection, resulting in a significant increase in the recruitment of hydrolytic enzymes by Brucella-containing phagosomes (BCPs) when IL10 signaling was blocked. Moreover, blocking IL10 signaling also enhanced proinflammatory cytokine production. Finally, concomitant treatment with STAT3 siRNA significantly reduced the suppression of proinflammatory brucellacidal activity but not phagolysosome fusion by rIL10. Thus, our data provide the first evidence that clearly indicates the suppressive role of IL10 on phagolysosome fusion and inflammation in response to B. abortus infection through two distinct mechanisms, STAT3-independent and-dependent pathways, respectively, in murine macrophages.
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Collections - 농업생명과학대학 > 축산과학부 > Journal Articles
- 수의과대학 > Department of Veterinary Medicine > Journal Articles
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