Unveiling the pathway to Z-DNA in the protein-induced B-Z transitionopen access
- Kim, Sook Ho; Lim, So-Hee; Lee, Ae-Ree; Kwon, Do Hoon; Song, Hyun Kyu; Lee, Joon-Hwa; Cho, Minhaeng; Johner, Albert; Lee, Nam-Kyung; Hong, Seok-Cheol
- Issue Date
- OXFORD UNIV PRESS
- NUCLEIC ACIDS RESEARCH, v.46, no.8, pp.4129 - 4137
- Journal Title
- NUCLEIC ACIDS RESEARCH
- Start Page
- End Page
- Left-handed Z-DNA is an extraordinary conformation of DNA, which can form by special sequences under specific biological, chemical or physical conditions. Human ADAR1, prototypic Z-DNA binding protein (ZBP), binds to Z-DNA with high affinity. Utilizing single-molecule FRET assays for Z-DNA forming sequences embedded in a long inactive DNA, we measure thermodynamic populations of ADAR1-bound DNA conformations in both GC and TG repeat sequences. Based on a statistical physics model, we determined quantitatively the affinities of ADAR1 to both Z-form and B-form of these sequences. We also reported what pathways it takes to induce the B-Z transition in those sequences. Due to the high junction energy, an intermediate B* state has to accumulate prior to the B-Z transition. Our study showing the stable B* state supports the active picture for the protein-induced B-Z transition that occurs under a physiological setting.
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- 자연과학대학 > 화학과 > Journal Articles
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