Generation and characterization of hagfish variable lymphocyte receptor B against glycoprotein of viral hemorrhagic septicemia virus (VHSV)
- Lee, Jung Seok; Kim, Jaesung; Im, Se Pyeong; Kim, Si Won; Lazarte, Jassy Mary S.; Jung, Jae Wook; Gong, Tae Won; Kim, Young Rim; Lee, Jeong Ho; Kim, Hyoung Jun; Jung, Tae Sung
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- PERGAMON-ELSEVIER SCIENCE LTD
- Variable lymphocyte B; Hagfish; Multivalent VLRB antibody; Epitope mapping; In vitro; Affinity maturation; Viral hemorrhagic septicemia virus (VHSV)
- MOLECULAR IMMUNOLOGY, v.99, pp.30 - 38
- Journal Title
- MOLECULAR IMMUNOLOGY
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- Variable lymphocyte receptors B (VLRBs) are non-immunoglobulin components of the humoral immune system in jawless vertebrates including hagfish (Eptatretus burgeri) and lamprey (Petromyzon marinus). Hagfish VLRBs consist of leucine rich repeat (LRR) modules with a superhydrophobic C-terminal tail, the latter of which leads to extremely low expression levels in recombinant protein technology. Here, we present an artificially oligomerized VLRB (arVLRB) that conjugates via the C4bp oligomerization domain derived from human C4b-binding protein (hC4bp) rather than the superhydrophobic tail. The resulting arVLRB had a tightly multimerized form with seven monomeric VLRB arms and showed high expression and secretion levels in a mammalian expression system. To isolate antigen-specific arVLRB, we constructed large VLRB libraries from hagfish immunized with the fish pathogen, viral hemorrhagic septicemia virus (VHSV). The selected arVLRBs were found to recognize various types of antigens, including the recombinant target protein, purified viruses, and progeny viruses, with high antigen binding abilities and specificities. We also performed in vitro affinity maturation of the arVLRBs through LRRCT mutagenesis, and found that this enhanced their antigen-binding properties by at least 125-fold. Our epitope mapping analysis revealed that (DWDTPL42)-D-37, which is located in a region conserved among the glycoproteins of all VHSV isolates, is the recognition epitope of the arVLRBs. Thus, our newly developed arVLRB could prove useful in the development of universal diagnostic tools and/or therapeutic agents for the virus. Together, our novel findings provide valuable insights into hagfish VLRB and its potential use as a novel alternative to conventional antibodies for biotechnological applications.
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- 수의과대학 > Department of Veterinary Medicine > Journal Articles
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