Effects of sodium hypochlorite on the potential infectivity of human norovirus GII.4 using propidium monoazide with RT-qPCR and quality assessments in Manila clams (Ruditapes philippinarum)
- Song, Min Gyu; Jeon, Eun Bi; Kim, Ji Yoon; Park, Shin Young
- Issue Date
- JOURNAL OF FOOD PROCESSING AND PRESERVATION, v.46, no.7
- Journal Title
- JOURNAL OF FOOD PROCESSING AND PRESERVATION
- This study investigated the effect of NaOCl on the inactivation of human norovirus (HNoV) GII.4 infectivity in Manila clams treated with NaOCl solution at 100-1000 mg/L chlorine for 10 min using propidium monoazide (PMA)/RT-qPCR. HNoV GII.4 detected using non-PMA/PMA were significantly (p < .05) reduced to 0.15/0.37, 0.33/0.58, 0.59/0.85, 0.69/0.99, and 1.05/1.54 log(10)copy number/mu l upon a 10 min treatment with 100, 300, 500, 700, and 1000 mg/L of NaOCl, respectively. The D-value of HNoV GII.4 infectivity in non-PMA and PMA-treated samples was calculated as 1000 and 714.3 mg/L of NaOCl, respectively, using the first-order kinetics model. The pH value increased significantly as chlorine concentration increased. Changes in color and appearance in quantitative analysis evaluation did not appear after treatment with 100-1000 mg/L chlorine although all sensorial parameters were above 4 points in all NaOCl treatments. The highest score of overall acceptability was observed in 100-500 mg/L chlorine-treated Manila clams. Practical applications The results suggest that PMA/RT-qPCR could help distinguish HNoV infectivity by negatively affecting clam flavor following NaOCl treatment with 1000 mg/L of chlorine. Moreover, the inactivation kinetics of chlorine against HNoV might provide further information for Manila clam processing and distribution.
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- 해양과학대학 > Seafood science & Technology > Journal Articles
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