Resistance to Brucella abortus 544 challenge in BALB/c mice by inoculation of highly immunogenic multiple B. abortus antigensResistance to Brucella abortus 544 challenge in BALB/c mice by inoculation of highly immunogenic multiple B. abortus antigens
- Other Titles
- Resistance to Brucella abortus 544 challenge in BALB/c mice by inoculation of highly immunogenic multiple B. abortus antigens
- Authors
- Lauren Togonon Arayan; Tran Xuan Ngoc Huy; Alisha Wehdnesday Bernardo Reyes; Vu Hai Son; 민원기; 이후장; 김석
- Issue Date
- 2019
- Publisher
- 한국예방수의학회
- Keywords
- B. abortus; cytokines; protection; recombinant proteins; immunization
- Citation
- 예방수의학회지, v.43, no.1, pp.1 - 5
- Indexed
- KCI
- Journal Title
- 예방수의학회지
- Volume
- 43
- Number
- 1
- Start Page
- 1
- End Page
- 5
- URI
- https://scholarworks.bwise.kr/gnu/handle/sw.gnu/10485
- DOI
- 10.13041/jpvm.2019.43.1.1
- ISSN
- 2287-7991
- Abstract
- In this study, we examined the protective immunity of a combination of seven Brucella abortus recombinant proteins; superoxide dismutase (rSodC), riboflavin synthase subunit beta (rRibH), 50S ribosomal protein (50s rL7/L12), nucleoside diphosphate kinase (rNdk), malate dehydrogenase (rMDH), arginase (rRocF), and elongation factor (rTsf) cloned in a pMal vector system and expressed in DH5α. Mice groups were immunized thrice with a combined subunit vaccine (CSV-7) at 0, 2, and 5 weeks and subsequently challenged with B. abortus at 5 × 104 CFU at 6 weeks. At four weeks post-infection, the mice were sacrificed and the bacterial burden in their spleens was quantified. Results revealed bacterial log reductions of 0.63 and 0.34 in comparison to PBS and maltose-binding protein (MBP), respectively. Cytokine profiling revealed a marked increase in IFN-γ (interferon-gamma), MCP-1 (macrophage chemoattractant protein-1) and IL-6 (interleukin 6) cytokines at 5-weeks post-immunization. On the other hand, only TNF was heightened at 7-weeks post-immunization. In general, this cytokine profile is consistently reflective of a Th1 immune response, which is beneficial for host immunoresistance.
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Collections - 수의과대학 > Department of Veterinary Medicine > Journal Articles
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