초미세먼지(PM2.5)로 유도된 in vitro 세포 독성에 대한 감태(Ecklonia cava) 추출물의 보호 효과

Abstract

To evaluate the protective effect of Ecklonia cava on ultra-fine dust (PM2.5)-induced cytotoxicity, we investigated the in vitro antioxidant activity and cell viability after exposure to PM2.5. E. cava was extracted using water and 80% ethanol, and antioxidant activity was determined using the 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS)/2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging and lipid peroxidation inhibition assays. The 80% ethanol extract showed relatively higher antioxidant activity than the water extract. The cell protective effects were determined by measuring the intracellular reactive oxygen species (ROS) content and viability of nasal epithelial (RPMI- 2650), lung epithelial (A549), and brain neuroblastoma (MC-IXC) cells. Results showed that the 80% ethanol extract inhibited ROS production more than the water extract. In contrast, both extracts showed similar effects on cell viability in the PM2.5-induced cell death assay. Thus, Ecklonia cava may act as an effective resource for preventing PM2.5-induced cytotoxicity in nasal, lung, and brain cells.