ScholarWorks@경상국립대학교

초록

Accurate and sensitive disease diagnosis is crucial to prevent the global spread of spring viremia of carp virus (SVCV). In this study, we developed and validated a novel dual-probe quantitative reverse transcription polymerase chain reaction (RT-qPCR) method, which detects two distinct SVCV genomic sequences. The analytical sensitivity of the developed method was 6.2 copies of the SVCV plasmid (LoD95%). No cross-reactivity was observed with other viruses and fish cell lines. The diagnostic performance was further assessed using 550 koi carp (Cyprinus carpio) with a confirmed SVCV infection status. Compared to the conventional RT-PCR method, the dual-probe RT-PCR method diagnostic sensitivity (Dsn) and specificity (Dsp) of 100 % and 100 %, respectively. Additionally, when tested with 266 koi carp samples with an unconfirmed SVCV infection status, the novel method demonstrated a Dsn of 100 % and Dsp of 54.5 %, when compared to the of conventional RT-PCR method. No significant differences in inter- and intra-laboratory reproducibility were observed when performing PCRbased diagnosis using the novel method. Overall, the dual-probe RT-qPCR method targeting SVCV was found to be reliable tool for detecting the virus, suggesting its potential use in quarantine, surveillance, and monitoring programs.

키워드