LPS에 유도된 RAW 264.7 세포에서 오미자(Schisandra chinensis ) - 토복령(Smilax china)의 항염증 효능 평가

초록

Purpose: This study investigated the anti-inflammatory effects of a Schisandra chinensis –Smilax china complex extract (SSCE) in lipopolysaccharide (LPS)-induced RAW 264.7 macrophages. Methods: SSCE was prepared from dried S. chinensi s and S. china using ethanol extraction. Cell viability of macrophages (RAW 264.7 cells) following SSCE treatment was assessed by MTT assay. Anti-inflammatory activity was assessed by measuring nitric oxide (NO) production using the Griess reagent, analyzing iNOS protein expression by Western blotting, and quantifying pro-inflammatory cytokine mRNA levels by reverse transcription-polymerase chain reaction (RT-PCR). Results: SSCE exhibited no cytotoxicity at concentrations of 25– 200 μg/mL in RAW 264.7 macrophages. Treatment with SSCE significantly reduced NO production and downregulated the expression of iNOS and pro-inflammatory cytokines, including tumor necrosis factor-α (TNF-α ), interleukin-1β (IL-1β ), and interleukin-6 (IL-6 ) in a concentration-dependent manner. Particularly, 200 μg/mL of SSCE inhibited NO production by 60.9% and iNOS expression by 69.8%. Moreover, SSCE suppressed TNF-α , IL-1β , and IL-6 mRNA expression by 68.6%, 75.7%, and 64.5%, respectively. Conclusion: These findings confirm that the S. chinensis –S. china complex extract imparts potent anti-inflammatory effects in LPS-stimulated macrophages. SSCE may therefore represent a promising natural candidate for the development of functional materials or therapeutic agents targeting inflammation- related diseases.

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